tobacco anti inflammatory Can nicotine improve cognitive function and inflammation in people with HIV? Fused EP 3: Smoking and

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The 1987 document explained the strategy like this

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At 80% confluency, cells were harvested using Dulbeccos phosphate buffered saline (DPBS) for washing and incubated with 0.05% trypsin EDTA/DPBS (GIBCO, Invitrogen, Carlsbad, CA) for 2 mins at 37 C to allow detachment from the culture flask