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Participants self-reported their cigarette and e-cigarette dependence at one, three and six months using validated measures of dependence, including a Penn State-developed questionnaire that ranges from 0 (not at all dependent) to 20 (highly dependent)

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From Santa Cruz Biotechnology (Santa Cruz, CA, United States), we purchased the mouse Abs against extracellular signal-regulated kinase (ERK) and phosphor-ERK (p-ERK)