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Brad Bird originally asked actress Lily Tomlin to do the voice of Edna, but when he showed her how he wanted Edna to sound, Lily Tomlin told him he should do the voice himself

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Therefore, further exploration of potential mitophagy regulatory factors (proteins or noncoding RNAs, etc.) is needed to uncover mitophagy mechanisms in diseases, providing a theoretical basis for further research on therapeutic strategies for these diseases