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Mechanistically, metformin may activate Nrf2 through multiple pathways, including AMPK activation, inhibition of GSK-3mediated Nrf2 degradation, and modulation of intracellular redox homeostasis, all of which facilitate Nrf2 nuclear translocation and subsequent induction of downstream antioxidant genes 48,49

Tatli YZ, Kapasi S

The pentose phosphate pathway generates NADPH from glucose, while mitochondrial enzymes produce NADPH to support glutathione recycling inside mitochondria [9]

If using bacteriostatic water is required, researchers must ensure the peptide solution is diluted significantly in culture medium to reduce benzyl alcohol below cytotoxic thresholds

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