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Finally, reads were mapped to the contigs using BWA-MEM to obtain read counts

Respiratory paralysis: Nicotine can suppress the signals that control breathing muscles, leading to respiratory arrest Severe and prolonged seizures: Extended convulsions cause physical injury and can result in permanent neurological damage Cardiac arrhythmias: Abnormal heart rhythms that destabilise circulation and can lead to cardiac arrest Severe hypotension: Blood pressure collapse that compromises blood flow to vital organs Coma: As the nervous system shuts down after the stimulation phase Death: In cases of large ingestion or delayed care, fatality is a real outcome The nervous system of a dog is not equipped to sustain the level of stimulation nicotine creates

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In the wake of the vaping epidemic, Michigan and New York have enacted laws banning the sale of flavored e-cigarettes, with the exception of tobacco and menthol flavored products