Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps
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Olsson CA, Coffey C, Toumbourou JW, Bond L, Thomas L, Patton G (2003) Family risk factors for cannabis use: a population-based survey of Australian secondary school students
If allowed to stand, this ruling would make it impossible to implement any effective warning labels, he said, adding that Leons ruling ignores the overwhelming scientific evidence about the need for the new cigarette warnings and their effectiveness and ignores First Amendment precedent that supports the right of the government to require warning labels to protect public health
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