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C57BL/6 mice were exposed to e-cigarette vapor (18 mg/mL nicotine content) for 5 days using a passive inhalation system mimicking real-life conditions (200 puffs/day, 3-second puffs, 1-minute intervals), while control mice were exposed to clean air, with experiments conducted one hour after the final exposure

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The regulations requiring the warnings on cigarettes were announced on May 31, 2023, with the implementation transition period that has since followed

The researchers were also able to trace the added cause of stress in the body and found marijuana boosted cortisol levels among marijuana addicts