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Ultrathin sections (80 nm thick) were cut with an ultramicrotome (Leica, EMUC6, Germany), and then stained with uranyl acetate and lead citrate and finally examined by a Tecnai G 2 Spirit TWIN transmission electron microscope (FEI, Hillsboro, OR, USA)

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When the conversion of propionyl-CoA to succinyl-CoA in the mitochondria fails due to Vitamin B 12 deficiency, elevated blood levels of methylmalonic acid (MMA) occur