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Assays and ELISAs for lung proteins quantification We used the proteins extracted from the lung samples (as described above) to perform a series of ELISAs or enzyme activity assays to determine the lung concentrations or activity of specific proteins involved in iron metabolism [ferroportin-1 (LS-F7200-1), transferrin (LS-F9542-1), ferritin light chain (LS-F8829), all from LS Bio, Shirley, MA, and lactoferrin (A74883, from Antibodies, St Louis, MO)], antioxidant response (NQO1 activity, AB184867, Abcam, Waltham, MA), and airway mucus hypersecretion (MUC5AC, LS-F4842, LS Bio, Shirley, MA)

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The 4-carton tier comes to $196, three dollars short of free shipping, which is why most regulars go to 5