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Mechanistic Studies Subsequent research has explored the mechanisms by which NNMT regulates metabolism, with studies demonstrating that NNMT activity reduces cellular NAD+ availability, affects SAM-dependent methylation reactions, modulates sirtuin activity, and influences adipocyte differentiation and function

Circulation 30:698705 Spirito P, Lupi G, Melevendi C, Vecchio C (1990) Restrictive diastolic abnormalities identified by Doppler echocardiography in patients with thalassemia major

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For the subsequent rounds of selection, crude phage supernatant rescued from bioassay plate outgrowths were used as the input for selection following the parameters described above