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HT29 cells were cultured in McCoys 5 A medium, and THP-1 cells were maintained in RPMI-1640 medium

In preclinical models, BPC-157 has been investigated for its influence on angiogenic signaling, nitric-oxidelinked pathways, and fibroblast activity associated with tendon and ligament biology [1][6][11]

Lai et al., 2009

Figure 5 In order to determine if the phosphorylation of Ser42, Ser184, and Tyr198 was involved in the effect of GSTP on HMGB1, six GSTP mutants were constructed, including GSTP-S42A (a Ser42 non-phosphorylatable mutant), GSTP-S42D (a Ser42 constant-phosphomimetic mutant), GSTP-S184A (a Ser184 non-phosphorylatable mutant), GSTP-S184D (a Ser184 constant-phosphomimetic mutant), GSTP-Y198F (a Tyr198 non-phosphorylatable mutant), as well as GSTP-Y198D (a Tyr198 constant-phosphomimetic mutant) (Figure S4B in Supplementary Material)

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