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Certain hotels, beach clubs, and restaurants may have designated smoking areas where vaping is permitted, but its always wise to ask first

Nuclear protein extraction HCE-T cells cultured in 60 mm dishes (Corning Inc.) were rinsed twice with ice-cold PBS and then harvested by scraping in the presence of 500 l of hypotonic buffer [20 mM TrisHCl (pH 7.4), 10 mM NaCl, and 3 mM MgCl 2 ] containing a protease inhibitor cocktail as well as phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich)

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