Select your preferred type and quantity below
2,7-dichlorofluorescin-diacetate (DCFDA) was used for reactive oxygen species (ROS) measurement
After blocking with Superblock T-20 (Thermo Scientific, Rockford, IL) membranes were incubated for overnight with primary antibody against GLUT1 for astrocytes and GLUT3 for neurons (11000, Abcam, Cambridge, MA) at 4C followed by 1 hr incubation with secondary antibodies conjugated with horse-radish peroxidase
To this extent, we studied the distribution of MZB precursors (MZP) in adult mice
After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)