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In the results of this study, the average levels of conventional chemical components in cigar tobacco samples from different regions of Yunnan generally fell within the appropriate ranges for each component, indicating a relative harmony in their chemical composition

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qPCR was performed using the SmartGene Sybr Green Q-PCR Master Mix with Low Rox (SMART GENE) according to the manufacturers instructions twice to determine the expression of IL-1, IL-6, TNF-, VEGF, and HIF-1