Burns' eldest sister, Agnes, lived in Dundalk from 1817 until her death in 1834 and was buried in the cemetery of St
Immunohistochemistry was performed as follows: free-floating VTA brain sections were incubated 1 h at 4 C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4 C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:500 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 (see supplementary Table 1)
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Stage 4a This stage is reached when the tumour is either very large (T4a) or has extended into the cervical lymph nodes (N2)
