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Gene functional annotation and GO analysis To investigate the global effects of the Nic1 , Nic2 , and Myc2a transcription factors at the root transcriptome level, all DEGs identified for comparisons between K326 and K326 nic1 / nic2 , K326 myc2a , and K326 nic1 / nic2 / myc2a were subjected to Gene Ontology (GO) enrichment analysis using AgriGO.v20 [43]