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It's generally recommended to avoid layering GHK-Cu with highly acidic ingredients like L-ascorbic acid (Vitamin C) or potent retinoids in the same session, as this can reduce efficacy or increase irritation

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doi: 10.1016/j.lfs.2022.120805 32

Secondary injuries are induced by a variety of physical and chemical factors such as local bleeding, edema, oxidative stress, and inflammation (Figure 3B)