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A sinusoidal voltage at the 5 kHz frequency was applied to the needle electrode, whereas the low voltage probe (Tektronix TPP1000) was adopted for collecting the current flowing through the sampling resistance (R = 1 k)

Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

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