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As our in vivo scRNA-seq data did not wholly capture the heterogeneity reflective of this progression, we studied the process by culturing basal tracheal epithelial cells at airliquid interface (ALI) and harvesting replicate cultures at 20 timepoints across mucociliary differentiation for scRNA-seq analysis (Fig

For the first-degree relatives of patients with IPF and no other known family members with ILD, however, there was disagreement about the balance of benefits versus potentially undesirable consequences

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