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For protein isolation, cells were washed with cold PBS, collected in lysis buffer using a cell scraper, incubated on ice for 20 min, and centrifuged for 5 min at 500 rpm

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After many years of footwork, on April 18, 2017, San Francisco City Supervisor Malia Cohen announced a proposed ordinance that would restrict the sale of any flavored tobacco product, including menthol, within the city limits