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Increased expression levels of genes associated with autophagy, oxidative stress and inflammation were observed in copper-treated HepG2 ATP7B-KO cells and in primary hepatocytes from patients with WD

Thats how it was discovered that levels were getting intracellularly and increasing

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The western blot results indicated that OGD and erastin caused a decrease in SLC7A11 and GPX4 protein levels, which were rescued with the addition of Fer-1 (Fig