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The studies analyzed consistently highlight adolescence as a critical period of neurobiological maturationparticularly within the prefrontal cortex and limbic regions that regulate cognition, emotion, and decision-makingduring which nicotine exposure may confer heightened vulnerability ( Nicotine acts on nicotinic acetylcholine receptors, affecting neurotransmission and synaptic plasticity
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Gas chromatography with a flame ionization detector (GC-FID) has been applied for the analyses of nicotine and artificial flavors in liquids and aerosols [42]
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