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ASK and VPPK carried out the metabolomic data analysis
Within biological systems, it is present in multiple forms, with six primary forms being pyridoxal (PL), pyridoxine (PN), pyridoxamine (PM), and their 5-phosphorylated derivatives: pyridoxal 5-phosphate (PLP), pyridoxine 5-phosphate (PNP), and pyridoxamine 5-phosphate (PMP) ( 6 , typically acts as a coenzyme and is involved in over 200 enzymatic reactions within cells, significantly contributing to the metabolism of proteins, fatty acids, and carbohydrates ( 6 de novo , rendering plants one of the primary sources of this vitamin in their diet ( In plants, pyridoxal 5-phosphate (PLP) is primarily biosynthesized de novo through the DXP-independent pathway, initially discovered in Arabidopsis thaliana ( de novo biosynthesis pathway of PLP, two glutamine transferases, PDX1 and PDX2, are required ( 6 via the salvage synthesis pathway, which is present in all living organisms ( A wealth of research has shown that genetic engineering techniques can successfully enhance the vitamin B 6 levels in plants
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