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Infection was induced through mechanical inoculation and leaves were harvested 2 weeks post-infection

A central component of the Study is upgrading the existing autoclave circuit to convert it to a modern POX circuit within the overall flowsheet design for improved operating efficiency (see Figure 8 in Appendix)

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This can cause parts of the leaf to be black from the process

Due to the simplification and improvement of aerosol formation modes, the properties and concentration of e-cigarette aerosol differ from tobacco smoke, further indicating that the toxicity of e-cigarette smoke is much lower than that of tobacco cigarette smoke [12]