In imagined time his course approached infinity, and during it he met other figures less palpable and far more real: his father jubilant at having his worst fears justified, his mother chalk-white on the sickbed to which his disgrace had brought her, the foul-mouthed trusty on his chain gang
Roth) diluted 1:100 in AP buffer (100 mM Tris-HCl, 100 mM NaCl, 5 mM MgCl 2 , pH 9.6)
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