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The ABCD study procedure was approved by the centralized institutional review board (IRB) of the University of California, San Diego, and by the IRB at each local institution

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Cigarette Smoke Extract ( CSE) was prepared as previously described with minor modifications 25 , and further diluted to the required concentration in serum-free medium

Consider these additional protective measures: Professional Cleanings Every 3-4 Months: More frequent visits help control plaque buildup Electric Toothbrushes: Remove more plaque than manual brushing alone Water Flossers: Effective for reaching deep periodontal pockets Vitamin C Supplements: Support gum tissue repair and immune function Sugar-Free Gum: Stimulates saliva production to neutralize harmful acids Is It Time for Professional Help