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Following washing, cortices were mechanically dissociated with repeated mixing using a 5 mL serological pipette and the resulting cell-suspension diluted with Opti-MEM (Life Technologies) supplemented with glucose (20 mM) and Antimicrobial-Antifungal to achieve a final tissue concentration of 0.14 mouse cortical hemispheres

Afaghi A, O'Connor H, Chow C: Acute effects of the very low carbohydrate diet on sleep indices

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Research has shown that it travels through the bloodstream to sites of injury, where it exerts potent anti-inflammatory and pro-regenerative effects

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