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Investigators are particularly interested in determining the mechanisms by which effects observed in murine models relate to broader mammalian metabolic regulation, and whether different experimental conditions (young vs aged models, healthy vs metabolically compromised states) show differential responses to NNMT inhibition

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TerpstraMCheongILyuTDeelchandDKEmirUEBednakPet al

After pharmacological intervention in 96-well cell culture plates for 24, 48 or 72 h, the culture medium was removed and replaced with 0.1 mg MTT reagent (Beyotime, ST1537) per well dissociated in 100 L basal DMEM (Procell, PM150210)

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