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Dose-Dependent Protective Effect of Nicotine in a Murine Model of Viral Myocarditis Induced by Coxsackievirus B3

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Along with primers and metagenomic DNA, a master mix was added containing dNTPs (0.5 mM), MgCl 2 (3.2 mM), high-fidelity DNA polymerase and PCR enzyme buffer