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CBD demonstrates both pro- and anti-inflammatory effects by ROS levels, and significantly attenuates LPS-induced NF-kB activity along with IL-8 and MCP-1

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15 To further verify whether the increase in cholesterol levels induced by cigarette smoke in airway epithelial cells contributes to cigarette smoke-induced airway inflammation through ROS promotion, we treated HBE cells with water-soluble cholesterol and detected ROS levels in airway epithelial cells using flow cytometry (Figure 6A) and the secretion levels of inflammatory factors IL6 and IL8 using qPCR (Figure 6B)