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Immunofluorescence staining for F-actin Cells were fixed using a fixative solution (2% paraformaldehyde + 2% glutaraldehyde in PBS) and permeabilized using 0.1% Triton X-100 in PBS, and F-actin and nuclei were stained using phalloidin-iFlour 555 (1:200, ab176156

A stark dictionary definition for "Cigarette" offers a cynical, humorous take: "A tiny, socially acceptable stick of self-destruction, guaranteed to make you smell like an ashtray and cough like a walrus." Presented in clean, minimalist typography, it's a dry, witty commentary on a common habit

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In the six years since he first appeared on YouTube, everything he's touched has taken on a sort of magical flyness