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Geotaxis assay In order to determine the appropriate concentration of e-nicotine for further experiments, a negative geotaxis assay, for the detection of the flies locomotory behavior against gravity, was performed

The CDC reports vaping is harmful to teens: Nicotine exposure during adolescent brain development can disrupt the growth of brain circuits that control attention, learning, and susceptibility to addiction

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Second, the variability in methodologies for measuring FMD also may limit the reliability of pooled effect sizes, as differences in occlusion cuff position and protocol could yield disparate results 30