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For use, aspirin and L-MS were dissolved in dimethyl sulfoxide (DMSO, #13445-74, Nacalai Tesque, Kyoto, Japan) to prepare stock solutions of 1 M and 250 mM These were further diluted in the culture medium for experimental use, ensuring a final DMSO concentration of 0.2% across all treatment and control groups

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Especially after the age of 25, it will drop significantly