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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection

The desired lipopeptide was obtained in 30% yield with 97% purity

Concurrently, the development and refinement of multicomponent quantitative fingerprinting strategies capable of capturing both chemical complexity and pharmacological consistency are urgently needed

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