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Studies have confirmed that cigarette extracts induce the conversion of macrophages to osteoclasts through the classic RANKL-RANK pathway, upregulate the expression of NFATc1, promote macrophage activation, and induce the expression of the osteoclastogenesis-related proteases TRAP, cathepsin K and MMP-9 (182)

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Luminex analysis A custom magnetic Luminex assay panel from Life Technologies was used as previously described 20 to assess the levels of the following 15 cytokines and chemokines in human plasma: IL-1, G-CSF, IL-10, IL-13, IL-6, IL-17, MIP1, VEGF, IFN, IL-12p70, IFN, IL-1RA, TNF, IL-4 and IL-8