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The biofilms in the wells were incubated at 37 C for 1 h, scraped thoroughly, and then 150 L solution from each well was transferred to a solid black microplate (PerkinElmer, USA) for top reading using a Varioskan TM LUX microplate reader (ThermoFisher, USA

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Researchers asked 291 smokers about their tobacco use patterns before and during the early months of the pandemic including how frequently they used tobacco products, reasons why their use patterns changed and whether they attempted to quit