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Participants were instructed to rinse with plain water before saliva collection to clear off food debris and waited for 10 min before starting sample collection to prevent sample dilution [13]

Withdrawal symptoms may also show up

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We estimate that, at the mean, the present value of the costs of this proposed rule is about $1.6 billion using a three percent discount rate and roughly $1.2 billion using a seven percent discount rate (2018$)