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ApoA1 protein expressed from ClearColi BL21(DE3) competent cells was Ni-column purified and tested for endotoxicity using both the HEK-Blue cell line from Invivogen (see Fig 5) and the LAL assay (see Fig
(2006) BioTechniques 41
Preparation of RNA from cultured adherent and suspension cells for RT-PCR
the key determinant of endotoxicity in eukaryotic cells
Lucigen EconoTaq DNA Polymerase
QuickExtract-FFPE DNA Extraction Kit, 50 mL size:EACH ApoA1 protein expressed from ClearColiSimple, rapid extraction of PCR ready DNA from challenging FFPE samples Fast: PCR ready DNA in minutes, not days Simple: No spin columns or transfer steps which helps increase yields Compatible: Extracted DNA is compatible with both real time and endpoint PCR Safe: No xylene or phenol extractions used The analysis of nucleic acid from formalin fixed, paraffin embedded (FFPE) specimens is challenging due to the extensive cross linking of all tissue
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