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A new metabolic marker for the (S)-mephenytoin hydroxylase, P4502C19

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To confirm that the effects observed by CBD in CYP-overexpressing microsomes are also observed in human liver, inhibition assays were performed in HLM, with similar IC 50,u values observed for CBD in HLM (0.98 0.28 M for cotinine formation and 0.50 0.11 M for nornicotine formation) as compared to that observed in incubations with microsomes from CYP2A6- or CYP2B6-overexpressing cells