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Southern Sub-Saharan Africa experienced the most significant reductions in ASR of deaths, DALYs, YLLs, and YLDs, with EAPCs of -2.15 (95% CI: -3.69 -0.58), -2.07 (95% CI: -3.03 -1.11), -2.2 (95% CI: -3.1 -1.29), and -2.01 (95% CI: -3 -1.01), respectively

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Thereafter, 50 l of MTT solution (Sigma-Aldrich, M5655, 5 mg/ml in PBS) was added into each well and cells were incubated at 37 for another 3 h

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