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A study conducted in 2016 revealed that mice exposed to benzopyrene exhibited higher levels of an immune receptor (AhR) in the epidermis, leading to an increase in the migration of immune cells (Langerhans cells), as well as levels of pro-inflammatory cytokines (IL-5, IL-13, and IL-17)

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Evidence of the potential cardiovascular harm of NTPs, especially e-cigarettes, comes from preclinical studies using animal models that mimic human usage during pregnancy, which have shed light on their detrimental health impact on fetuses and children/offspring who are involuntarily exposed