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We implemented a systematic approach to select our aimed sample of 900 students, ensuring an equal representation across different academic years

For each replicate: (1) for determination of nicotine, 0.1 mL of the MTBE phase was placed in a ~ 2 mL brown glass autosampler vial with a septum cap then diluted using 0.9 mL MTBE

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The secondary container must be firmly and snugly packed within a strong outer shipping container that is securely sealed