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If a second primary antibody was used, the cells were incubated either for 2 h at room temperature or overnight at 4C

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The statistical software "Primer of Biostatistics, version 3.02" was used

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Consistent with these findings, physiological measurements demonstrated that melatonin continued to improve airway function in the presence of Luzindole ( Figures 2O, P ), suggesting that receptor-independent mechanisms may contribute to melatonins protective effects in allergic airways disease