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Immediately upon incubation completion, 10 l Proteinase K (20 g/l) and 50 l 10% SDS were added to the tubes and incubated at 55C for 30 minutes
Akishima S, Matsushita S, Sato F, Hyodo K, Imazuru T, Enomoto Y, Noma M, Hiramatsu Y, Shigeta O, Sakakibara Y: Cigarette-smoke-induced vasoconstriction of peripheral arteries: evaluation by synchrotron radiation microangiography