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The limit of quantitation was administratively set at 10 ng/mL and signal-to-noise ratio was greater than 10 times the baseline

2.5 Nicotine quantification by LC-MS Liquid chromatography was conducted before mass spectrometric analyses using an Agilent HPLC System Series 1100 (Agilent Technologies, Waldbronn, Germany) connected to a HCT Ultra PTM Discovery System (Bruker Daltonics, Bremen, Germany) with electrospray ionisation (ESI) and ion trapping

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