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Drugs that target the TLESRs also have been used, including baclofen, which has been shown to reduce the total number of gastroesophageal reflux events, but its use in clinical practice is limited by its lack of tolerability

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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Even vegetables like sweet potatoes and squash can be problematic