DNA Fingerprinting size:For 6 groups of 4-5 or 24-30 students. the strains can be differentiated

the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector

The following laboratory activity is adapted from “Laboratory 4h: DNA Extraction from Bacteria” from Biotechnology: Laboratory Manual by Ellyn Daugherty

bacterial colonies can be grown on solid agar plates to show the vast numbers of bacteria present in the mouth

 The basic principle of the PCR reaction is to heat the DNA to be copied to >90ºC to denature it

Electroelution is routinely used to extract both nucleic acids and proteins from electrophoretic media

DNA Fingerprinting size:For 6 groups of 4-5 or 24-30 students. the strains can be differentiatedTodays media routinely covers forensic stories of criminals captured and innocents released using the powerful DNA fingerprinting technique. This kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo crime scene. Students will use specialized molecules to cut the DNA into fragments, which can then be visualized by a process known as electrophoresis. The resulting