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Poly (A) purification
Volume: 25ml
High transformation efficiency with clones of all sizes
Ideal Use: Cell culture
660 mM potassium acetate
DNA Ligation size:For 6 groups of 4-5 or 24-30 students. Poly (A) purificationFollowing restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyzes the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose
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