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Apart from the GSH-recycling method, GSSG is generally reduced to GSH by thiol exchange type reagents (like dithiothreitol) or by phosphines and then the thiol group is labeled as mentioned above

Serum IGF-1 and BALP were measured using sandwich ELISA with serum IGF-1 ELISA (DRG International, USA) and Microvue BALP ELISA (Quidel Corporation, CA, USA) kits respectively

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Therefore, we replaced the ketone in ThiolQuant Green with a less reactive amide in RT to balance the K d back into the mM range

reduced glutathione (GSH) is the active form that is able to repair oxidative damage, and oxidized glutathione (GSSG) is the inactive form that can be recycled back into GSH