Pay in 4 interest-free payments of $135.38 Learn more
Ships within 48 hours · Estimated delivery Aug 14 - Aug 19
Contaminating DNA fragments left behind by these methods ultimately can become ligated into a cloning vector
Quality Control: T4 PNK is tested in 5´ phosphorylation of nucleic acids and is free of detectable exo- and endonuclease and RNase activities
65°C for 60sec
40-mer oligonucleotides with (5´-PO4) or without (5´-OH) phosphorylated 5´-termini were incubated with the indicated number of units of T4 PNK in 1X T4 PNK Reaction Buffer for 16 hours at 37°C
Quality Control: Exo-Minus Klenow DNA Polymerase (D355A
HI-Control-BL21(DE3) Chemically Competent Cells, 12 rxns (SOLOs) TransforMax EPI300 Contaminating DNA fragments left behindHI Control BL21(DE3) and HI Control 10G Competent Cells Induce high level protein expression from lac based promoters (T7, T5 lac, tac, trc, lac) Tightly regulate expression from T7 promoters with HI Control BL21(DE3) Chemically Competent Cells Clone targets and reduce background protein expression with HI Control 10G Chemically Competent Cells The HI Control strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a